Custom peptide synthesis
Phosphorylated Peptides
Our expertise in Post-Translational Modifications (PTMs) such as phosphorylation spanning several years has helped in the research of phosphopeptides for several important biological applications.
Our chemists can incorporate single, or multiple combinations of phosphoserine (-pS-), phosphotyrosine (-pY-), or phosphothreonine (-pT-) residues into your custom peptide.
Typical location of phosphoryl groups
The addition of a phosphoryl group can occur on Serine (Ser), Threonine (Thr) and Tyrosine (Tyr) side chains via a phosphoester bond formation.
Specifications
Length : Up to 60 amino acids
Purity : >95%, >90%, >85%, >70% or crude
Quantity : 1 mg minimum up to gram quantities, delivered as gross weight or net weight.
Applications
- Development of phospho-specific antibodies
- Mass standards for proteomic identification
- Assay development as phosphatase substrates
- Mapping and Quantifying Phosphorylation sites
- Cellular signaling/signal transduction pathways
- Studying structural PTMs that regulate protein function.
- Studying roles in disease pathologies including cancer, neurodegenerative and metabolic disorders.
Featured Citations
Quantitative Analysis of ATM Phosphorylation in Lymphocytes.
Bakkenist CJ, Czambel RK, Lin Y, Yates NA, Zeng X, Shogan J, Schmitz JC.
DNA Repair. 2019 Aug 1;80:1-7
From AnaSpec : Purified peptides SLAFEEGSQSTTISSLSEK(S1981) and SLAFEEG pS QSTTISSLSEK(p-S1981)
Regulation of folate and methionine metabolism by multisite phosphorylation of human methylenetetrahydrofolate reductase.
Zheng Y, Ramsamooj S, Li Q, Johnson JL, Yaron TM, Sharra K, Cantley LC.
Scientific reports. 2019 Mar 12;9(1):1-1
From AnaSpec : Kinase substrate library
About Phosphorylation
Phosphorylation and dephosphorylation of proteins are critical processes to the proper regulation of signaling pathways that control basic cellular functionality. Abnormal phosphorylation and dephosphorylation are both implicated in many diseases including, cancer, diabetes, Alzheimer’s and cardiac disease.
Aberrant protein phosphorylation in different brain regions is considered a key pathogenic event in the progression of Alzheimer’s disease. Hyperphosphorylated Tau is observed in Alzheimer’s brain which acquires resistance to dephosphorylation by protein phosphatases thereby contributing to further protein aggregation.
Inappropriate kinase activity/expression alters the signal to cause abnormal cellular function leading to different types of cancers. Varied expression of kinases in tumor samples have highlighted the need to identify them as biomarkers for cancer.
Kinase inhibitors in therapeutic development serve as prime candidates in the development of new cancer therapies.
Complex peptide synthesis
We deliver up to grams of peptides in research grade or as critical raw material.