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Assay-kits

SensoLyte® AMC Calpain Activity Assay Kit Fluorimetric - 1 kit

$586.00
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  • Cat.Number : AS-72150
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The calpains are a family of intracellular Ca2+ dependent cysteine proteases. The best-characterized calpains are isoforms 1 (m) and 2 (m). Calpains respond to Ca2+ signals by cleaving many specific proteins, thereby irreversibly modifying their function(s). They are implicated in a variety of Ca2+ regulated cellular processes as well as various pathological phenomena, such as ischemic injury, muscular dystrophy, diabetes, cataract, atherosclerosis, Alzheimer’s disease, and cancer. Calpains represent potential therapeutic targets for drug discovery.
The SensoLyte® AMC Calpain Assay Kit is optimized for detecting calpain activities. This kit contains a fluorogenic peptide substrate, suc-LLVY-AMC. Upon calpain protease cleavage, suc-LLVY-AMC generates the AMC fluorophore emitting bright blue fluorescence and can be monitored at excitation/emission=354/442 nm. Increase in AMC fluorescence is proportional to calpain activity. The assay can detect both calpain 1 (µ) and 2 (m) activities and is ideal for kinetic study of these enzymes. The assays are performed in a convenient 96-well microplate format.

Specifications

Packaging
Kits components
  • Component A: Calpain Substrate, Ex/Em=354 nm/442 nm, upon cleavage: 4 mM, 50 µL Component B: AMC, Fluorescence Reference Standard, Ex/Em=354 nm/442 nm: 4 mM, 10 µL Component C: Assay Buffer: 20 mL Component D: Human Calpain: 1.25 mg/mL, 40 µL Component E: Calpain Inhibitor: 50 µM, 10 µL Component F: DTT: 1M, 20 µL
Chemistry
UniProt number
  • P07384
Properties
Absorbance (nm)
  • 354
Emission (nm)
  • 442
Storage & stability
Storage Conditions
  • Store all kit components, except Component D, at -20°C. Store Component D at –80°C. Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture. Component C can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 156 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Genetic myostatin decrease in the golden retriever muscular dystrophy model does not significantly affect the ubiquitin proteasome system despite enhancing the severity of disease

Am J Transl Res. . 2013 Dec 01 ; 6(1) 43 | DOI : PMC3853423

  • S.W. Cotten
  • et al

Calpain mediates epithelial cell microvillar effacement by enterohemorrhagic Escherichia coli

Front Microbiol . 2011 Jul 27 ; 2 222 | DOI : 10.3389/fmicb.2011.00222

  • Y.S. Lai
  • et al

Regulation of the calpain and ubiquitin-proteasome systems in a canine model of muscular dystrophy.

Muscle Nerve . 2011 Aug 08 ; 44(4) 553 | DOI : 10.1002/mus.22125

  • K.M. Wadosky
  • et al