Assay Kits by Disease/Research

Assay kits detection methods

FRET Protease assay

• Fluorescence or Förster resonance energy transfer (FRET) is a distance- dependent transfer of excited state energy from an excited donor (fluorophore) to an acceptor (another fluorophore or quencher). This transfer prevents the fluorophore to emit a fluorescent signal at its emission wavelength. Consequently these assays measure events allowing the physical separation of the fluorophore and the quencher, such as proteolytic cleavage, leading to the release of the fluorescent signal.
• Fluorogenic peptide protease assays also measure the activity of proteases but a fluorogenic peptide is used as a substrate.
  
  

ELISA

During Enzyme-linked immunosorbent assay (ELISA), an unknown amount of target is specifically immobilized on a solid support (capture of target antibody by a specific antigen; capture of target protein by a specific antibody). Detection is performed using a labeled antibody conjugate, which produces a signal (fluorimetric, colorimetric or luminometric), proportionnal to the quantity of tested compound in the sample.

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FRET + ELISA

FRET technology combined with ELISA assay allows a specific detection of the activity of a particular protease in a biological sample, possibly containing multiple proteases which recognize the same FRET peptide-substrate. The target protease is selectively captured by ELISA, then its activity is assayed using a FRET peptide.

FRET Dye & Quencher couples

Full spectrum of dyes and quenchers covering all detection channels