Convenient

Our Assay kits are convenient for general research or high throughput screening for the discovery of enzyme inhibitors or enzyme activators.

Sensitive

Our FRET assay kits and Fluorogenic assay kits are highly sensitive with high signal-to-background ratios and are tested for quality.

Adaptable

Ourhomogenous mix-and-readenzyme activity kitsare available as off-the-shelf catalogproducts but can also be optimizedfor your specific application. Bulk quantities available.

Our collection of Activity Assay Kits

We offer sensitive activity kits to easily detect protease and enzyme activity. Our Assay kits give fast results with very high S/N ratio. Our line of SensoLyte® Protease and Enzyme Activity Assay Kits are ideal for high throughput screening drug discovery of enzyme inhibitors and enzyme activators.

AnaSpec also offers a variety of highly sensitive non-protease enzyme activity assay kits including those for Sirtuins, HDAC, Glutaminyl Cyclase, Pin1, Phosphatases, Peroxidases and Galactosidase.

Category Products
Acetylcholinesterase SensoLyte® 520 Acetylcholinesterase Assay Kit Fluorimetric Order Now
ADAM SensoLyte® 520 ADAM10 Activity Assay Kit Fluorimetric Order Now
SensoLyte® 520 TACE (a-Secretase, ADAM17) Activity Assay Kit Fluorimetric Order Now
ADAMTS SensoLyte® 520 ADAMTS13 Activity Assay Kit Fluorimetric Order Now
SensoLyte® 520 Aggrecanase-1 Assay Kit Fluorimetric Order Now
ACE 2 (Angiotensin-Converting Enzyme 2) SensoLyte® 390 ACE2 Activity Assay Kit Fluorimetric Order Now
Calpain SensoLyte® 520 Calpain Activity Assay Kit Fluorimetric Order Now
SensoLyte® AMC Calpain Activity Assay Kit Fluorimetric Order Now
Caspases SensoLyte® Caspase Activity Assay Kits Order Now
Cathepsins SensoLyte® Cathepsin Activity Assay Kits Order Now
Deubiquitination SensoLyte® 520 Deubiquitination Assay Kit Fluorimetric Order Now
SensoLyte® 440 Deubiquitination Assay Kit Fluorimetric Order Now
Dipeptidyl Prolyl Isomerase 4 (DPPIV) SensoLyte® Rh110 DPPIV Assay Kit Fluorimetric Order Now
SensoLyte® AMC DPPIV Assay Kit Fluorimetric Order Now

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Category Products
Elastase SensoLyte® Green Elastase Assay Kit Fluorimetric Order Now
SensoLyte® Rh110 Elastase Assay Kit Fluorimetric Order Now
ECE (Endothelin Converting Enzyme) SensoLyte® 520 ECEs Activity Assay Kit Fluorimetric Order Now
Enterokinase SensoLyte® 520 Enterokinase Assay Kit Fluorimetric Order Now
SensoLyte® Rh110 Enterokinase Assay Kit Fluorimetric Order Now
Factor Xa SensoLyte® 520 Factor Xa Assay Kit Fluorimetric Order Now
SensoLyte® Rh110 Factor Xa Assay Kit Fluorimetric Order Now
Furin SensoLyte® Rh110 Furin Activity Assay Kit Fluorimetric Order Now
GBA (Glucocerebrosidase) SensoLyte® Blue Glucocerebrosidase (GBA) Activity Assay Kit Fluorimetric Order Now
SensoLyte® Red Glucocerebrosidase (GBA) Activity Assay Kit Fluorimetric Order Now
Glutaminyl cyclase SensoLyte® Green Glutaminyl Cyclase Activity Assay Kit Fluorimetric Order Now
Granzymes A & B SensoLyte® 520 Granzyme A Activity Assay Kit, Fluorimetric Order Now
SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric Order Now
Glutathione-S-Transferase (GST) SensoLyte® GST Activity Assay Kit Fluorimetric Order Now

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Category Products
HDAC (Histone deacetylase) SensoLyte® 520 HDAC Activity Assay Kit Fluorimetric Order Now
HAT (Histone acetyltransferase) SensoLyte® HAT (p300) Assay Kit Fluorimetric Order Now
HCV Protease SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays Order Now
SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 200 assays Order Now
SensoLyte® 520 HCV Protease Assay Kit Fluorimetric Order Now
HIV Protease SensoLyte® 490 HIV Protease Assay Kit Fluorimetric Order Now
SensoLyte® 520 HIV Protease Assay Kit Fluorimetric Order Now
Insulin Degrading Enzyme (IDE) SensoLyte® 520 IDE Activity Assay Kit Fluorimetric Order Now
Kallikrein SensoLyte® Rh110 Plasma Kallikrein Activity Assay Kit Fluorimetric Order Now

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Category Products
Matriptase SensoLyte® Rh110 Matriptase Activity Assay Kit Fluorimetric Order Now
Matrix Metalloproteinases (MMPs) SensoLyte® MMP Activity Assay Kits Order Now
Meprins SensoLyte® 520 Meprin β Activity Assay Kit Fluorimetric Order Now
SensoLyte® 520 Meprin α Activity Assay Kit Fluorimetric Order Now
Neprilysin SensoLyte® 520 Neprilysin Activity Assay Kit Fluorimetric Order Now
Peptidyl-Prolyl Isomerase (Pin1) SensoLyte® Green PIN1 Activity Assay Kit Fluorimetric Order Now
Peroxidase SensoLyte® Peroxidase Assay Kits Order Now
Phosphatase SensoLyte® Phosphatase Assay Kits Order Now
Plasmin SensoLyte® AFC Plasmin Activity Assay Kit Fluorimetric Order Now
SensoLyte® Rh110 Plasmin Activity Assay Kit Fluorimetric Order Now
Plasminogen Activator SensoLyte® AMC Tissue-type (tPA) Activity Assay Kit Fluorimetric Order Now
SensoLyte® AFC Urokinase (uPA) Activity Assay Kit Fluorimetric Order Now
Protease-Generic SensoLyte® Green Protease Assay Kit Fluorimetric Order Now
SensoLyte® Red Protease Assay Kit *Fluorimetric Order Now

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Category Products
Renin SensoLyte® Renin activity assay kits Order Now
SARS-CoV-2 Proteases SensoLyte® SARS-CoV-2 3CL Protease Activity Assay Kit Fluorimetric Order Now
SensoLyte® SARS-CoV-2 Papain-like Protease (PLpro) Activity Assay Kit Fluorimetric Order Now
Secretases SensoLyte® 520 β-Secretase (BACE1) Activity Assay Kit Fluorimetric Order Now
SensoLyte® 520 BACE2 Activity Assay Kit Fluorimetric Order Now
SensoLyte® 520 TACE (a-Secretase) Activity Assay Kit Fluorimetric Order Now
Sirtuin (SIRT) SensoLyte® 520 FRET SIRT1 Assay Kit Fluorimetric Order Now
SensoLyte® Green SIRT1 Assay Kit Fluorimetric Order Now
SensoLyte® 520 FRET SIRT2 Assay Kit Fluorimetric Order Now
SensoLyte® Green SIRT2 Assay Kit Fluorimetric Order Now
Sortase A Sortase A Order Now
Thrombin SensoLyte® 520 Thrombin Activity Assay Kit Fluorimetric Order Now
SensoLyte® AFC Thrombin Activity Assay Kit Fluorimetric Order Now
Tobacco Etch Virus (TEV) Protease SensoLyte® 520 TEV Protease Assay Kit Fluorimetric Order Now
Transglutaminase SensoLyte® Red Transglutaminase Assay Kit Fluorimetric Order Now
SensoLyte® Transglutaminase Activity Assay Kit Colorimetric Order Now
West Nile Virus (WNV) Protease SensoLyte® 440 West Nile Virus Protease Assay Kit Fluorimetric Order Now
SensoLyte® 570 West Nile Virus Protease Assay Kit Fluorimetric Order Now

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FRET substrates

FRET occurs between a peptide tagged to a donor and an acceptor when placed within 10-100Å of each other resulting in the donor’s excitation fluorescence to be quenched by the acceptor. Enzymatic hydrolysis of the peptide results in recovery of the donor fluorescence following spatial separation of the donor and acceptor upon energy transfer.

Dye (donor) Quencher (acceptor) Donor Ex/Em
SensoLyte® 390 Mca Dnp

 325/393 nm

SensoLyte® 490 EDANS DABCYL

 340/490 nm

SensoLyte® 520 5-FAM or HiLyte™ Fluor 488 QXL®520

 494/521 nm

SensoLyte® 570 5-TAMRA QXL®570

 547/574 nm

 

Fluorogenic substrates

These substrates do not require a quencher and contain a C-terminal dye that does not fluoresce until it is cleaved from the peptide (fluorescent form of dye is released).

Dye (donor) Donor Ex/Em
SensoLyte® AMC AMC

 351/430 nm

SensoLyte® AFC AFC

 382/480 nm

SensoLyte® Rh110 Rh110

 501/527 nm

Why is Protease Activity Important?

Protease enzymes catalyze the breakdown of proteins into smaller peptide fragments and/or single amino acids. Endopeptidases are proteases that cleave internally of the protein sequence, and those that cleave at the N-terminus or C-terminus are exopeptidases.

Endopeptidases are categorized into 4 classes according to the catalyst type (amino acid or metal) that is located within the active site that drives the hydrolytic breakdown of the substrate peptide bond, including (a)Serine Proteases (Trypsin, Elastin, Thrombin, HCV NS3/4A), (b)Cysteine proteases (Caspase, Cathepsin), (c)Aspartyl Proteases (HIV, Renin), and (d) Metalloproteases (MMPs).

Endopeptidases

Through very specific and controlled protein substrate cleavage, proteases help in the initiation, modulation and termination of many crucial cellular functions, including DNA replication, cell proliferation, wound healing, immunity, angiogenesis and apoptosis. Proteases are involved in many diseases caused by aberrant metabolic protease activity or through infection by pathogens that express proteases for their infectivity or replication

Role of Proteases

Activation of Bid in apoptosis by Caspases-8, Calpains & Cathepsins B, L S, H, D, acting via the loops or interdomain linker regions.

Processing antigens via the lysosomal system for presentation by MHC class II molecules, such as cathepsins and deubiquitinating enzymes.

Ectodomain processors by exposing cryptic sites, such as beta-secretase during amyloid precursor protein processing or Shedding various transmembrane proteins such as metalloproteases and cysteine proteases.

ECM modulators by using exosites as effective substrate binding, such as thrombin, ADAMTS-4 and most MMPs.

Targets for viral inhibitors to arrest viral proteases processing viral polyproteins in causing pathogenicity